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SuperMICAR was designed to facilitate the entry of literal text of causes of death in death certificates and convert them into standardized expressions acceptable by MICAR.
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Since the expression levels for each gene is standardized to have mean 0 and standard deviation 1, the standardized expression values fall within [-3, 3].
To determine whether the WRKY expression profiles were correlated with flag leaf development, NMDS ordination based on a Bray-Curtis dissimilarity matrix of the standardized expression levels of the differentially expressed (DE WRKYY genes in switchgrass flag leaves was performed.
Genes that were significantly differentially expressed were binned into classes using conditional statements, considering standardized expression levels and the statistical relationship between contrasts of interest.
ACORNS Effect Size is a standardized expression of the effect of the curriculum mini-unit for a particular teacher.
GAENE Effect Size is a standardized expression of the effect of the mini-unit for a particular teacher.
A standardized expression value (D statistic = coefficient/ (error + median error of all genes), [46]) was then calculated for each gene in the experimental and the permuted data sets.
For clarity in the figure, NF1 was represented as "NF1 loss" (zero minus standardized expression), reflecting the role of NF1 as an inhibitor of RAS signaling.
All standardized expression data were then log-transformed to normalize the residuals in order to meet the necessary assumptions for the statistical tests (after normalization, the residuals did meet these assumptions).
The Fourier score is defined as
For each dataset, a gene expression matrix with normalized and standardized expression values was thus generated.
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