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Yeast biofilms were formed in the wells of microtiter plates by pipetting standardized cell suspension of freshly grown and washed yeast cells into wells of microtiter plates and incubating at 37°C (9 ).
ONS cells are standardized cell populations grown under strict quality assurance such that technical variables are small compared with individual patient and donor variability (Mar et al., 2011; Matigian et al., 2010).
Analysis of data collected by standardized cell isolation methods and data reporting requirements readily identified methodological problems, indicating that standard reporting parameters facilitate post hoc error identification.
Their goal is to facilitate the engineering of more differentiated cell sheet products such as muscle tissue or cartilage, and enable higher throughput generation and testing of multiple standardized cell sheets in parallel.
Because of the lack of a standardized cell culture method, real-time reverse transcriptase PCR is now commonly used for the detection of NoV in foodstuffs and environmental samples.
In order to develop suitable bioreactor systems and processes for automated and standardized cell cultures involving the use of bioactive factors, we determined the stability of transforming growth factor beta 3 (TGFβ3) over storage time and under conditions typically used for mammalian cell culture.
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In order to obtain comparable data, it is important to standardize cell culture methods.
To establish an ACCS for iPS cells in a limited space, it is necessary to standardize cell culture operations.
After standardizing cell samples to one million in different groups using a cell counter, 0.5 mg/ml 3- 4,5-Dimethylthiazol-2-yl -2,5-dippphenyltetrazolium bromide (MTT) was added to chondrocytes incubated at 3- 4,5-Dimethylthiazol-2-yl -2,5-dippphenyltetrazolium
Islet cell autoantibodies are not recommended for routine diagnosis of diabetes, but standardized islet cell autoantibody tests may be used for classification of diabetes in adults and in prospective studies of children at genetic risk for type 1 diabetes after HLA typing at birth.
ATP levels were standardized to cell number for isolated hepatocytes or protein levels using BCA assay (ThermoScientific, Rockford, IL, USA) for cultured cells.
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