It is important to standardize protocols for blood collection, PBMC isolation, cryopreservation, and thawing that maximize survival and functionality of PBMC at the time of analysis.
Today, no generally accepted standardized protocol for assessment of ex vivo TNF-α rexists exists, hindering multicenter studies [46].
Implementation of this rational, standardized protocol for the assessment and treatment of stable adult patients presenting with acute undifferentiated febrile illness can lead to reduced rates of testing and antimicrobial use.
Implementation of a rational, standardized protocol for the assessment of stable adult patients with acute undifferentiated febrile illness in this south Indian emergency department demonstrates a potential to lower rates of unnecessary testing and antimicrobial use.
Pulsed-field gel electrophoresis was performed according to the PulseNet standardized protocol for V. cholerae (14 ).
Since PFGE is a gel-based method, it requires strict adherence to standardized protocol for reproducible results.
Biopsies were separately scored by two pathologists blinded to the clinical data, according to the previously standardized protocol for scoring renal biopsies of patients with AAV [ 25– 27].
However, all centers used a uniform and standardized protocol for data collection and the process of gathering all available information was rigorously applied.
Pulsed-field gel electrophoresis was performed according to the PulseNet standardized protocol for V. cholerae (www.pulsenetinternational.org/SiteCollectionDocuments/pfge/5.71_2009_PNetStandProtVcholerae.pdf. Gel Compare II software (Applied Maths NV, Sint-Martens-Latem, Belgium) was used for comparison of electrophoresis patterns.
In the present study, we optimized an effective, minimally disruptive, and standardized protocol for whole-liver decellularization in a large animal model that produces a human-scale three-dimensional liver matrix suitable for supporting functional hepatocytes.
