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Therefore, it is important to minimize technical sources of variation with rigorous attention to detail in the standardization of processing pipelines, including automation and consideration of the use of microfluidics approaches which have the additional advantage of reduced reaction volumes (19).
However, the selection of less drastic fixatives, and the improvement and standardization of processing procedures, may preserve nucleic acids, and possibly proteins in order to integrate new techniques to routine surgical pathology determinations.
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A step in addressing standardization of tissue processing is to document how different labs process their samples.
In addition, different methodologies and lack of standardization in processing cost make comparisons between countries difficult.
A standardization of data processing and protocols for 18F-FET-PET is needed in order to make clinical results comparable.
A standardization of data processing and evaluation is needed to make 18F-FET PET comparable between different centres.
However, standardization of SWI processing is necessary to compare results between studies.
Standardization of specimen processing and the establishment of a comprehensive quality control program make the GEP70 assay highly suitable for the routine diagnostic clinical setting.
Moreover, standardization of sample processing procedures and use of the technology, and data analysis and algorithms used are of critical importance.
These can be confounding factors when analyzing miRNA expression from tissue specimens collected in different institutions and at different times, especially in historical archives dating back to the 1980s and 90s, before standardization of tissue processing procedures.
However, standardization and centralization of processing at a dedicated single site bring benefits in robustness of the data trail, reduced cost and increased achievable throughput and accuracy of sample handling and picking; for example, through the use of automation.
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