There is no evidence of standardization of PRP preparation and use [ 16] but common clinically available materials for blood preparations combined with a two-step low force centrifugations, to ensure cellular component integrity, provided good platelet preparations [ 17].
Nevertheless, standardization of drugs preparation and educational tool improved nurses' knowledge and developed a real team awareness about drugs PCI.
Another challenge is the lack of standardization of sample preparation strategies for lipid analysis, which normally includes at least one liquid/liquid extraction step in order to remove lipids from the complex biological matrix.
Analytic improvements are likely to improve diagnostic accuracy but require such strict standardization of patient preparation, scan protocols, and image reconstruction that separate studies in each PET centre are warranted to establish relevant threshold values.
The absence of an internal standard spiked early in sample preparation protocols means that label-free methods are sensitive to technical variance, consequently, label-free proteomics requires high instrument performance and standardization of sample preparation methods.
Despite this difficulty, standardization of patient preparation, scan acquisition, and quality control are becoming components of many current PET studies, improving quality and contributing to more reproducible PET results.
Findings from these studies and the results from this current study suggest a clear need for the standardization of the preparation of protease containing allergen extracts from Af and other fungi.
However, despite the important technological progress made in the past years in this field, measurement of B- and T-cell responses still faces many difficulties: standardization of cell preparation, timing of blood draw, cells conservation, reproducibility and inter-laboratory comparability, available volumes of blood in the pediatric population, and assay costs.
Standardization of sample preparation protocols should increase reproducibility of these studies, and will make it easier to select candidate genes and proteins that are suitable for validation as biomarkers for either fibrosis staging, or as predictive biomarkers for disease outcome and/or treatment response.
These contrasting observations of the antimicrobial properties of GO may be due to the lack of standardization of GO preparations, producing particles with different sizes and numbers of sheets.
