Sentence examples for standardization expression from inspiring English sources

For standardization, expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (TaqMan; Applied Biosystems) was quantified for each sample [ 18].

A qRT-PCR reaction for the exogenous RCP1 control gene was performed to allow for standardization of expression of the CF genes by accounting for differences between samples in the RT reactions.

Another important point for future studies is standardization of NKLR expression, for example by normalization according to standard cells, or by employment of a different method such as quantitative real time PCR.

Standardization in gene expression analysis is of central importance.

Standardization of gene expression data from three biological replicates was performed as described [ 74].

Briefly, standardization of gene expression data was performed from three biological replicates as described [ 106].

The SPEX system consists of three major components: (i) embryo standardization, (ii) gene expression pattern extraction and (iii) feature representation.

For standardization of gene expression levels, mRNA ratios relative to RP29 as housekeeping gene were calculated, and real-time quantitative data were analyzed using the ΔΔCt method.

After standardization of the expression values from the different time points, clustering by complete linkage based on centered correlation was carried out.

Each sample was tested in triplicate with quantitative PCR and, for standardization of gene expression levels, mRNA ratios relative to the house-keeping gene GAPDH were calculated.

We conclude that such prototype reference panels could be useful QC materials for the standardization of gene expression measurements between laboratories and platforms, and in aiding interpretation of biomarker profiling data in regulatory settings.