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Furthermore, the test scores were standardised using a mean value of 500 and a standard deviation of 100.
Measured fluorescence intensities were standardised using a fluorescein-specific fluorescence calibration kit (Sphero FITC Calibration Kit, Spherotech, # ECFP-F1-5K).
In Germany, 361 of the participants were measured using a GAITRite® walkway system and were standardised using a stopwatch.
Participants are encouraged to complete repetitions consecutively and verbal motivation is standardised using a protocol during all testing sessions.
The foot position was standardised using a pre-formed triangle (heels together with feet forming an angle of 20 degrees).
The analysis of this data was standardised using a structured coding model based on the study objectives and recurrent themes in the literature.
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The expression levels of the target miRNAs were standardised using an index containing 5S rRNA and U6 snRNA.
The interviewer probes were standardised using an interview topic guide based on the four-stage model of question response proposed by Tourangeau: comprehension, retrieval, judgement and response [ 19].
Data were standardised using GAPDH as a housekeeping gene.
Expression of each gene was standardised using GAPDH as a reference, and relative levels in LoVo, L2 and L3 cells were quantified calculating 2ΔΔ C T, where ΔΔ CT is the difference in CT (cycle number at which the amount of amplified target reaches a fixed threshold) between target and reference, relative to parental LoVo cells.
Each of these variables was standardised using the formula: standardised value= value−mean)/standard deviation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com