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At each follow-up visit, CD4 cell count was measured by standardised flow cytometry, and plasma HIV RNA levels were measured using the assay routinely available in each participating centre.
Thus, we linked prescription and administration by a standardised flow of information to the prescribing physicians and investigated its benefit.
In contrast, a standardised flow cytometry protocol for mHLA-DR measurement has been established so as to obtain reproducible results between centres.
The European Research Initiative on CLL have proposed an international standardised flow cytometric approach (ISA) and compared it against real-time quantitative allele-specific oligonucleotide immunoglobulin heavy chain gene PCR.
The admitting medical staff used a standardised flow sheet (Additional file 2: Table S2) to allocate patients to each category from the information available from relatives or the medical records.
Recommended actions and practice changes included clinical, laboratory and preventive strategies and target setting for chronic illnesses, standardised flow sheets, vigilant monitoring of prescription renewals for patients on long-term medications, and ensuring continuity of care for patients seen in on-call and episodic circumstances.
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The fluorescence was evaluated using a EPICS® XL-MCL™ flow cytometer (Beckman Coulter Inc., Brea, California, USA), and fluorescence intensity was standardised using Flow-Set fluorospheres (Beckman Coulter Inc., Brea, California, USA).
After an additional washing step (PBS, twice, 5 min, 1000 rpm) the expression of ICAM-1 and VCAM-1 was analysed using the EPICS® XL-MCL™ flow cytometer (Beckman Coulter Inc., Brea, California, USA), and fluorescence intensity was standardised using Flow-Set fluorospheres (Beckman Coulter Inc., Brea, California, USA).
To standardise the flow cytometry results, calibration particles (Rainbow Beads; Spherotech Inc., Libertyville, IL, USA) were measured in each sample run.
Our findings may help develop temporal, seasonal and weather corrections to standardise cycle flows in future surveys which are mostly conducted at a specified time during the year.
FLM structuring operations are limited to metering, mixing, deposition and fusion; while materials used in FLM fabrication must have highly-standardised flow and setting properties.
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