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Inactivated human serum samples were pre-incubated with a standardised amount of virus prior to the addition of MDCK cells.
Flies were infected at the same time of day with a standardised amount of C. albicans yeast cells administered by the same person.
Secondly, the energy needed to create the fracture- is not perfectly controlled when considered in comparison with a dropping tower, which delivers a highly standardised amount of energy.
A standardised amount of virus (100 infectious Unit [TCID50] in 0.05 mL) was mixed with serial dilutions of sera and incubated to allow binding of the antibodies to the virus.
Firstly, the General Directorate of Pharmaceutical and Health Products (DGFPS) of the Valencian Health Department designed a standardised amount indicator [ 34] which offered primary health care pharmaceutical expenditure data per patient covered in a year.
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The endogenous reference gene GAPDH was used to standardise the amount of sample RNA.
We didn't standardise the amount of force used for measurement of passive RoM.
In order to standardise the amount of RNA in each sample, β-actin mRNA was used as an internal control.
Food hoppers were also exchanged during baseline and replacement phases to standardise the amount of disturbance each day.
Amplification of the house-keeping gene TATA-Box-binding-Protein (TBP, Tbp) was performed to standardise the amount of sample RNA.
The defined daily doses (DDDs) recommended by the World Health Organization (WHO ATC/DDD index database) were used to quantify and standardise the amount of NSAID usage.
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