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Culture strains of E. coli and S. aureus (Mc Farland 0.5 standard) were prepared.
All methanolic working solutions of analytes and the internal standard were prepared by serial dilution of stock solutions.
Stock solutions of each antineoplastic drug, including that of the internal standard, were prepared in methanol at 400 μg/mL.
For the quantitative analysis, six standard stock solution (Stock B and Stock C) containing different concentration levels of each identified analyte and the same amount of internal standard were prepared.
Formulations containing Amphotericin B based on selected top recipes and the standard were prepared as above, but with 10uM calcein in the hydration buffer.
Different serial dilutions of the standard were prepared.
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An international radium standard was prepared in 1911 by Mme.
This internal standard was prepared sterile at one time as follows.
The standard was prepared by incubating shrimp oil at 35°C for 0, 5, 10 and 15 days.
An internal standard was prepared containing a mixture of equal amounts of proteins from each extract.
The standard was prepared using the AmBisome recipe [10], [11], but with our protocol.
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