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Working solutions 0.1 mg · mL-1 of each standard were made in 0.1% ammonium formate in 10% acetonitrile (buffered at pH 5.5) and from this, the calibration standards dilutions between 5 μg · mL-1 and 0.005 μg · mL-1 were freshly prepared before each analytical series.
Two additions of a Fluka analytical standard were made to the sample and the concentration was determined using the standard addition method.
To evaluate LPS contamination in the isolated EVs, PBS- and LPS-induced EVs (5 μg) or different doses of LPS, as a standard, were made to react with LAL assay kit QCL-10000; Lonza, Basel, Switzerland) following an instruction manual.
For the quantitation of quercetin in plasma stock solutions (10 mg ml−1) of quercetin and kaempferol (internal standard) were made up (DMSO), and the calibration curve was constructed with five concentrations of quercetin (final concentrations 1, 2, 20, 100 and 200 μg ml−1).
Similar(56)
Another much-cited ruling that used the voter-intent standard was made by the Massachusetts Supreme Judicial Court in 1996.
"However, there is no evidence from which this panel may determine that a finding of 'no fault' under the current WADA standard was made or could be inferred".
A side-by-side drawdown of the sample and standard was made.
Accordingly, a provisional mass standard was made as a single-piece, metallic artifact one thousand times as massive as the gram the kilogram.
Protein standard was made using 2 mg/ml bovine serum albumin (Sigma-Aldrich).
0.5 mL of the protein standard was made up to 50 mL of 0.85% sodium chloride solution.
An internal, arbitrary standard was made of a mixture of total RNAs from PBMCs taken from three healthy donors.
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