Exact(31)
The reaction was stopped with 1 mL of methanol and 30 μL 1 N HCl before 500 μL PBS and 200 ng PGB1 (internal standard) were added.
Spiked samples were prepared in the same steps, except a known amount of ractopamine standard were added to the pork sample before treatment.
Spiked samples were prepared in the same steps, except a known amount of CLP standard were added to the pork samples before treatment.
Reaction mixture and internal standard were added to each cell extract and transferred to a thermal cycler.
Then, 25 µl sample or standard were added with 200 µl working reagent in duplicates to a microplate.
Briefly, 96-well microplates were coated with native HBs and after washing and blocking steps, dilutions of serum samples, controls and standard were added to the plate.
Similar(29)
Five-hundred picoframs of 13C6-IAA internal standard was added to each sample before purification.
Methanol:toluene 2 ml (4:1 v/v) (containing C19 0 (20 μg/ml) as internal standard) was added to the sample.
In this case, no reference standard was added.
A standard concentration of 5 µg/ml SOR (internal standard) was added to the solutions.
The standard was added to the organic nitrogen compound-enriched fraction prior to GC-MS analysis.
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