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Besides the standard strands, there were noodles cut in small triangles and noodles shaped like worms with pointed ends.
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The oxoguanine glycosylase activity was measured using the standard strand-nicking assay.
By using the standard strand displacement assay the helicase activity of OsSUV3 was determined using the partially duplex hanging tails substrate which consisted of a P-labelled 47-mer DNA oligodeoxynucleotide annealed to M13mp19 phage ssDNA.
Mr. Fagin, an experienced environmental reporter who now teaches journalism at New York University, could have braided together three or four standard narrative strands into a perfectly serviceable book, starting with factory air so polluted it dissolved the nylon stockings off women's legs and ending with dying children, anguished parents, industrial cover-ups.
A five-strand braid looks a little more intricate and elegant than a standard three-strand braid, and it's easy to do once you get the process down.
Standard second-strand synthesis was followed by standard library preparation with the double-stranded cDNA as input material.
The script then trimmed off the forward primer sequence and oriented the remaining sequence such that all sequences begin with the 5' end according to standard sense strand conventions.
After purification standard double stranded adapters from the Roche 454 kit were ligated with NEB Quick Ligase, following Roche 454 Titanium protocols.
The standard DNA strand-displacement assay was used in order to characterize the DNA unwinding activity of OsSUV3.
Our previous protocol was also used to select high-quality 16S rRNA gene amplicon sequences (with >350 and <550 bases in lengths) and for their subsequent processing (i.e. alignment to a standard sense strand and removal of primer sequences for pyrosequencing).
Finish with a standard 3-strand braid.
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