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Cellulose-bound peptides were prepared according to the standard SPOT synthesis protocol by a MultiPep SPOT-robot (INTAVIS Bioanalytical instruments AG, Köln, Germany) on a β-alanine-modified cellulose membrane as described elsewhere [ 26].
The peptides were automatically prepared onto Amino-PEG500-UC540 cellulose membranes according to standard SPOT synthesis protocols [ 31] using an Auto-Spot Robot ASP-222 (Intavis Bioanalytical Instruments AG, Köln, Germany).
Successfully matched spots were compared across groups according to SA (standardized abundance), where a sample spot fluorescence volume (Cy5) is divided by the standard spot volume (Cy3), normalized with ratiometric normalization and transformed with a log10 function.
And there's no standard connector in a standard spot, so there's no universe of docks, alarm clocks, car adapters as there is with the Apple gadgets.
The standard spot for prospective chief executives at major oil companies is the exploration and production division, which is usually the big profit earner.
Standard spot return/forward premium regressions have long been known to provide a strong rejection of unbiasedness.
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Advertisers can integrate themselves into skits and bits as well as run standard spots.
I took cheapo hometown friends there for pre-marathon carbo-loading when more standard spots were booked, and we loved the homey service and freshly made pasta.
Based on the fluorescence intensities of artificial standard spots, the raw fluorescence intensity data for specific spots could be corrected to generate linear correlations with target concentrations.
These data describe the degree of variability that may be associated with bone lead measurements of young subjects with low bone lead concentrations using a standard spot-source KXRF instrument.
Calibration was performed using default calibration originated by five standard spots (ABI4700 Calibration Mixture).
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