Standard protocol involved 125 mL of iopamidol injected at 4 mL/sec.
The standard protocol involved 35 1 h sessions (at least 5 times/week), but some failed to complete the whole course treatment for reasons as described below.
All operations were performed according to a standard protocol involving the posterior approach, paying special attention to the excision of all abnormal metal-contaminated tissue.
These profiling experiments used U95Av2 GeneChips (Affymetrix, Santa Clara, CA) and two different methods for target preparation, namely, a standard protocol (involving in vitro transcription, IVT; [ 7, 8]) and an amplification protocol (involving double in vitro transcription, dIVT; [ 7, 9]).
Due to intrinsic genetic instability and hypersensitivity towards DNA crosslinking agents in FA, treatment options are limited, excluding standard protocols involving cisplatinum.
This was treated by standard protocols involving hospital admission, intravenous antibiotics and (if required) the use of granulocyte-colony-stimulating factors.
The standard wash protocol involves five 300 μL washes, no soaking, with a crosswise aspiration after the final wash.
The standard hemodynamic protocol involves maintaining the mean arterial blood pressure (MAP), but in patients with intracranial hypertension it may not be enough to maintain adequate cerebral perfusion.
The standard NCS protocol involves sensory NCSs of the lower extremity sensory nerves, mainly the sural and superficial peroneal nerves, along with motor NCSs of the peroneal and tibial nerves.
One key difference of the protocol described in the Materials and Methods section that we used to generate gene expression profiles from low amounts of RNA is that we performed three consecutive linear amplification steps, whereas the standard Affymetrix protocol involves only one linear amplification step.
One hundred and eighty-two participants underwent a standard laboratory stress protocol, involving a 20-min baseline rest period, and four stressors presented in a counterbalanced order, each followed by a 10-min recovery period.
