Sentence examples for standard cycling from inspiring English sources

Standard cycling parameters (35 cycles) were used for the qualitative RT PCR analysis on P1- and P2-MDM2 transcript expression.

PCRs were pursued in triplicate by using a StepOnePlus Real-time PCR system (Applied Biosystems), and a standard cycling profile of 45 cycles in a volume of 25 µL containing random hexamer-primed cDNA, 300 nmol/L primer (each), and 200 nmol/L probe.

We used the ABI 7900 machine with the standard cycling protocol, increasing the number of cycles to 40.

The reactions (final volume of 25 μl) were set with a QuantiFast SYBR Green PCR Master Mix (Qiagen) together with forward and reverse primers (10 mM; 2.5 μl each, see Additional file 2: Table S2) and amplified using standard cycling conditions: 95°C (10 min) and 40 cycles of amplification (95°C for 30 s and 60°C for 1 min).

Limitation of lithiation or delithiation to an intermediate value of 900 mAh g−1 allows to perform up to 1850 cycles at C/5 rate, which represents a significant increase of the electrode cycle life compared to that observed for standard cycling.

Standard cycling parameters were: 94°C for 3 minutes, 35 cycles of 94°C for 50 seconds, locus-specific annealing temperature for 50 seconds, 72°C for 50 seconds, and a final extension of 72°C for 5 minutes.

Methods: In 6 patients under PSV, IntelliSync + was compared to standard cycling-off with both default setting (ETS = 25%% of peak inspiratory flow) and optimized setting guided by bedside waveforms analysis of patient-ventilator interaction (PVI).

A standard PCR cycling program with 35 cycles at low stringency (annealing temperature 45°C) with primers specific for different A. asiaticus 5a2 IS elements was used for the detection of IS elements in the A. asiaticus strains EIDS3, WR and US1 (see Additional file 2, Table S1 for primer sequences).

Real-time qPCR was performed in triplicate (96-well plates) on an ABI 7900 (Applied Biosystems) machine using standard thermal cycling conditions (10 min at 95°C, 40 cycles for 15 s at 95°C, 1 min at 6°C).

Standard thermal cycling conditions (10 min at 95°C, 40 cycles for 15 s at 95°C, and 1 min at 60°C) were used for all genes.

Performing a standard thermal cycling reaction at 58°C annealing with 35 cycles of amplification produced detectable levels of PCR product of the expected size for all of the LS primer sets tested, confirming the specificity of the amplifications.