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Prior to zeta potential analysis, standard control samples were run on the instrument.
For standard control samples, 1.5 mL TiO x solution was spin-coated onto a cleaned fluorine-doped tin oxide (FTO) substrate with the same area at 3000 rpm for 30 s.
Briefly, 100 μl of sample and standard control samples provided with the assay were incubated on microtiter plates that were pre-coated with a monoclonal antibody specific for CAIX.
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A "standard" control sample was chosen to run on each gel.
A standard control sample was included in each run to correct the curve for run-to-run variation.
Human postmortem brain samples were run on multiple gels, each of which contained a standard control sample to enable comparison of samples across gels.
For immunofluorometric measurement of Mcm5 levels, assay standards, control samples and gastric aspirate samples were run as duplicates and the mean of the duplicate results reported.
For immunofluorometric measurement of Mcm5 levels, assay standards, control samples and bile aspirate samples were run as duplicates and the mean of the duplicate results reported.
Each reaction included a standard-control sample and a non template control.
To quantify absorbance values, we performed the same procedure for standard and control samples provided by the manufacturer, and created a standard curve.
To quantify the absorbance values, we performed the same procedure for standard and control samples provided by the manufacturer, and created a standard curve.
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