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We only used genes from standard chromosomes (no genes from random or haplotype-specific chromosomes).
Date of origin was estimated from the observed number of segregating sites within inverted and standard chromosomes as described [30].
Within the inversion at locus 2110, diversity was reduced nearly 19-fold on inverted versus standard chromosomes.
The estimate is based on the observed number of segregating sites within the inverted and standard chromosomes at a locus inside the inversion adjacent to the breakpoint, where neither crossing over nor gene conversion are expected [31].
This could be an indication of positive selection operating in these regions on standard chromosomes.
However, the differences among sites within chromosome arrangements were small compared to the differences between inverted and standard chromosomes.
Similar(34)
Engineered minichromosomes are small chromosomes that contain a transgene and selectable marker, as well as all of the necessary components required for maintenance in an organism separately from the standard chromosome set.
Interestingly, the mosquitoes that failed to show an effect of LRIM1 knockdown on P. falciparum infection [28] all carried the standard chromosome arrangement (2L+).
Under this scenario, the starting point would have been a single 14.6 kb LCR at the proximal breakpoint on the standard chromosome.
First, if both 14.6 kb LCRs were already present at both future breakpoints on the standard chromosome, these nearly identical sequences could serve as targets for non-allelic homologous recombination, leading to inversion of the intervening sequence.
This duplication originally would have existed as a short direct repeat flanking the proximal 14.6 kb LCR on the standard chromosome, and may have resulted from double-strand break repair upon its integration.
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CEO of Professional Science Editing for Scientists @ prosciediting.com