Sentence examples for standard assay procedures from inspiring English sources

The residual lipase activity of the samples was then assayed under standard assay procedures.

The optimum temperature for the purified lipase was measured at various temperatures 20-50°C 20-50°C standard assay procedunder

The optimum pH for the purified lipase was evaluated over a pH range from 6.0 to 11.0 under the standard assay procedures.

The remaining activities were measured as the standard assay procedures using pNPG as the substrate, and the control enzyme activity without cations was taken as 100%.

We used standard assay procedures, using undiluted eluates from oral swabs and DBS; improvements to sensitivity may be possible with further optimization of the serological and NS1 antigen assays for oral swab samples.

The effects of various metal ions such as K+, Cu2+, Co2+, Zn2+, Ni2+, Cd2+, Ca2+, Mn2+, Mg2+, Ba2+, and EDTA were tested under the standard assay procedures, with the addition of each ion at a concentration of 5 mM.

Protease activity was determined by following the standard assay procedure.

Protein concentrations were determined by the standard assay procedure using Pierce Coomassie® Plus Protein Assay Reagent.

Kinetic parameters (Km and Vmax) were determined by the measurement of activity against pNPX using different substrate concentrations (0.5 - 12 mM) using the standard assay procedure.

The reaction mixture was incubated at respective temperatures for 30 min before determining protease activity according to the standard assay procedure earlier described.

Effect of inhibitors (phenylmethylsulphonyl fluoride [PMSF], β-mercaptoethanol [β-ME] and ethylene diamine tetra acetic acid [EDTA]) at 5 mM on protease activity was determined by pre-incubating the purified enzyme solution with inhibitor for 30 min at 40°C before the addition of substrate following the standard assay procedure.