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Following addition of internal standard, as above, oxysterols were extracted from the medium into 2-propanol:hexane (40 60, 9 mL, 50 μg/mL BHT) [25].
The extinction coefficient (2970 M/cm) of released pNP at pH 6.0 at 37°C was determined using the same standard as above.
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All data were collected using standard definitions as above, using a standardised electronic spreadsheet developed with the Clinical Research Support Centre (CRSC), in which tick boxes are used to record each defined variable within predefined ranges.
Formulations containing Amphotericin B based on selected top recipes and the standard were prepared as above, but with 10uM calcein in the hydration buffer.
The method was validated in blood using solvent and matrix standard curves prepared as above.
TBARS were determined fluorometrically (λexcitation=515 nm; λemission=553 nm) and expressed as nmol malondialdehyde (MDA) by comparison with a standard (1,1,3,3-tetraethoxypropane 1,1,3,3-tetraethoxypropane
The same gloss standard as mentioned above is used for the calibration of the handheld glossmeter, and similar semiconductor laser serves as the light source.
The result is compared with an external reference standard, as discussed above.
The expression levels of each protein were determined by densitometry, after correction for protein loading using Ku80 as an internal standard, as described above.
One hundred and forty-one function-specific networks were learned with identical structures, each using a subset of the global gold stanetworks described above.
In all laboratories, serum was thawed at room temperature and combined with an internal standard, as described above in "Round Robin Design"; note that different standards were available during different phases of the Round Robin.
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