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Five methods: the Bootstrap, the Jackknife, Log-likelihood Profiling, Case-deletion Diagnostics and Stepwise Covariate Model building are included as separate classes and may be used in user-written Perl scripts or through stand-alone command line applications.
For blast screens, we employed the stand-alone command line version of NCBI blast 2.2.27+ (ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/, last accessed on November 11th, 2014) [ 74].
Availability and implementation: crAss is available as a web server at http://edwards.sdsu.edu/crass/, and the Perl source code can be downloaded to run as a stand-alone command line tool.
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The tie-points reduction algorithms are implemented as stand-alone commands within the MicMac tool.
Most analysis functions are implemented by stand-alone command-line programs.
The stand-alone, command-line program was written using Perl language.
Each module works independently as a stand-alone command-line program, with configurable settings for contingencies of data quality, experimental design and primers.
These stand-alone commands that are executed like common UNIX commands leverage third-party JSON libraries to process JSON strings.
Source code and stand-alone compiled versions of command-line and easy-to-use graphical user interface (GUI) versions of RF++ for Windows and Linux as well as a user manual (Supplementary File S2) are available for download at: http://sourceforge.org/projects/rfpp/ under the GNU public license.
As a stand-alone entity, Chrysler shouldn't command such a sum.
OligoArray 2.1 [ 4, 5], GoArrays [ 6], OligoPicker [ 7], ArrayOligoSelector [ 8], YODA [ 9], ProbeSelect [ 10], and Picky [ 11] are stand-alone tools that run in command mode and provide unique probe candidates for input sequences under the constraints of GC%, Tm, absence of low complexity, and position near the 3' end.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com