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Both stains were visualized by goat anti mouse Alexa Fluor 488 (Invitrogen, Molecular Probes) at a concentration of 1∶1000.

Stains were visualized by 3,3-diaminobenzidine (Dako), with Mayer's hematoxylin (Sigma) as a counter stain, and mounted.

Histological stains were visualized by fluorescence microscopy on an AX70 (Olympus) and digital images were captured and processed using Magnafire (Optronics) and Photoshop (Adobe) software.

Staining was visualized by Zeiss microscope and analyzed by Image-Pro plusoftwarere.

Staining was visualized by 3,3'-Diaminobenzidine.

Actin staining was visualized by fluorescent microscopy at 40X magnification.

Islet staining was visualized by using a nickel/cobalt enhanced diaminobenzidine solution.

Subsequently the staining was visualized by applying ABC complex and DAB solution.

Staining was visualized by 3-amino-9-ethylcarbazole (AEC) followed by a hematoxylin (Sigma, St . Louis MO) nuclear counterstaining.

Positive staining was visualized by applying 3-amino-9-ethylcarbazole (AEC, Sigma); nuclei were counterstained with haematoxylin.

Staining was visualized by incubation with Alexa-Fluor secondary conjugates (Invitrogen) for 10 minutes at room temperatures.