Hemotaxlyin and Eosin (H&E) staining of light microscopic sections was used to quantify the amount of damage the muscle experienced due to EP.
Alcian blue staining showed a general trend of light staining in the middle with stronger staining along the periphery of the pellet for B, C and NCA media groups; however, NCA demonstrated darker staining along the periphery.
The mean staining intensity was estimated and expressed as weak (yellow or light-brown cytoplasmic or nuclear staining), moderate (dark-yellow or medium-brown staining, or a mixture of light and dark staining) or strong (dark-brown staining in nucleus or cytoplasm).
The colocalization with ryanodine receptors is highly incomplete, which is again consistent with CA IV staining of the light SR in addition to staining of the terminal SR.
In contrast, no evident differences in fast muscle fibers in the morphants were identified via the staining of myosin light chains with an F310 antibody (Fig. 4A, i l).
In contrast, treatment with 17-AAG diminished the cytoplasmic staining of lambda light chain with the majority of the staining localized to the peri-nuclear region.
Staining of the light microscopic sections with Toluidine Blue clearly demonstrated that the central vacuoles in the adaxial epidermal cells of the inner petal region could contain up to several highly condensed 'cores' within an AVI (Fig. 5A, white arrowhead).
The intensity of staining were recorded on a scale of 0 (no staining), 1 (weak staining, light yellow), 2(moderate staining, yellowish brown) and 3 (strong staining, brown).
The intensity of staining was recorded on a scale of 0 (no staining), 1 (weak staining, light brown), 2 (moderate staining, yellowish brown) and 3 (strong staining, brown).
The intensity of staining was recorded as following: 0 (no staining), 1 (weak staining, light yellow), 2 (moderate staining, yellowish brown), and 3 (strong staining, brown).
