Sentence examples for stains fixed from inspiring English sources

Cells were washed of excess surface stains, fixed and permeabilized using Cytofix/Cytoperm™ (BD Biosciences), and stained using anti-IL4 (11B11, BD Biosciences) in permeabilization buffer (FA Buffer+0.1% saponin (Sigma)) at 4°C for 30 mins.

For actin staining, fixed cells were incubated with TRITC-conjugated phalloidin (Sigma).

The cells were stained, fixed in 2% paraformaldehyde and analyzed by flow cytometry.

Where indicated, 10× SYBR Green I (Molecular Probes, Eugene, OR) instead of HO was used to stain fixed cells.

Compensation samples were prepared by staining fixed hESCs with APC-conjugated mouse anti-human SSEA4 antibody, PE-conjugated mouse anti-human SSEA4 antibody (both from R&D Systems Inc).

First, as a control, we confirmed that the mRFP signal represented APP by counter-staining fixed transfected/HSV1-infected cells with anti-APP antibodies.

In the remaining half-aliquots, cells were counted (trypan blue staining), fixed, and transfection efficiency was evaluated by means of a flow cytometer.

For β-gal/LacZ staining, fixed cells/sections were incubated in 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal) solution at 37°C for 24 hours.

To visualize the luminal secretion of those O-glycans in the salivary gland, we stained fixed embryos with the specific Tn antibody.

MΦs were stained, fixed, and permeabilized according to manufacturer recommendations, followed by incubation with diluted mAb to V for 1 h at 37°C (5% CO2).

Terminal adipocytic and osteoblastic differentiation was assessed by staining fixed cells with 0.5% Oil Red-O (Sigma) and 1% Alizarin Red S (Sigma), respectively.