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All negative controls were done using the same staining without using primary antibodies.
Complement 3 glomerulopathy (C3GN) is a newly proposed subcategory of glomerular disease with features including membranoproliferative glomerulonephritis (MPGN), C3-dominant immunofluorescent staining without appreciable immunoglobulin deposition, and electron-dense deposits.
Notably, no dyes for long-term staining without cytotoxic side-effects have been found; the field is dominated by DNA-staining dyes, which has cytotoxicity as an intrinsic limitation.
The system produces robust and consistent staining, without causing tissue degradation or blotchy artifacts.
Staining without applying a first antibody served as negative control.
Simultaneous staining without primary antibodies excluded false positive results.
hs-APPL-FLAG transgenic larvae also showed no staining without heatshock (Figure 6B, H, N).
MAbs were selected simply for positive staining, without any discrimination towards stronger or weaker staining.
Isotype controls and staining without primary antibody were used as controls.
Sorting gates for the gp41 MPER+ fractions were set to include all events greater than baseline staining without tetramer.
Compared to the control staining without any peptide (left panel) the incubation with peptide alone revealed only a marginal increase in peptide signal (middle panel).
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