In the second set, an optimisation of the de-staining protocol step with respect to the length of the de-staining procedure was implemented to prevent avoidable background staining, and thus inadequate staining results.
Therefore, staining for p63, α-SMA and PAS was implemented to aid in the evaluation of invasive regions.
Gating was implemented based on negative control staining profiles.
For the separation, gating was implemented based on the negative-control staining profile of MSCs in direct coculture with EPCs.
Finite element modeling, using the commercial Finite Element program FlexPDE (PDE Solutions Inc., 2006), was implemented in order to solve the governing partial differential stress-stain equations for underground pipes.
A similar type of workshop was implemented for the first time in chemistry in 2012 (Baker et al. 2014, Stains et al. 2015).
Options specifically for predicting archaeal proteins and proteins in organisms with membrane structures not reflecting Gram stains have also been implemented.
Compared with these massive gene signature-based models (Kwon et al, 2004; Fritzmann et al, 2009; Watanabe et al, 2009), the IHC staining was easily to be implemented and our IHC-SVM arithmetical approach might to be a useful decision-support tool in future clinical practice.
