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Cytoplasmic staining was ignored.
Concomitant cytoplasmic staining was ignored because HIF-1α protein is functionally active in the nucleus.
Similarly, HIF-2 α staining was predominantly nuclear and occasional focal cytoplasmic staining was ignored.
HIF-1α staining was considered positive only when there was homogeneously dark nuclear staining; cytoplasmic staining was ignored.
Furthermore, cytoplasmic staining was ignored as imaging antibodies will not be easily internalized and will have to bind to receptors on the outside of the cancer cells.
Similar(55)
Only membrane staining was scored, with cytoplasmic staining being ignored.
FOXP3+ cells with moderate or high intensity (++ or +++) were considered Tregs while weak staining were ignored.
However, cytoplasmic staining was observed similar to that reported earlier for placental extravillous trophoblast and lung cancer, but was found to be nonspecific background staining and was ignored (Sarhadi et al, 2006).
Staining of mitotic figures was ignored and only nuclear staining was regarded as positive for P53.
Any staining of the tumour stroma was ignored in this assessment.
Cytoplasmic staining of HIF-1α, observed occasionally, was ignored.
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