SA-β-gal staining was conducted after the seventh subculture.
Nissl staining was conducted to observe the neuronal degeneration and wet-to-dry weight ratio indicated brain water content.
Cartilage staining was conducted as described [33].
Immunocytochemical staining was conducted as described [8].
β-Galactosidase staining was conducted as described previously [25].
Wheat Germ Agglutinin (WGA) staining was conducted with WGA-Alexa Fluor 488 conjugate (1∶250).
Both primary and secondary staining was conducted for 30 minutes at room temperature in FACS buffer.
Immunohistochemical staining was conducted using a DAB system (Zymed Laboratories INC). in accordance with the manufacturer's instructions.
After electrophoretically transferring the proteins to a nitrocellulose membrane, a Panceau S staining was conducted to visualize the proteins.
FOXP3 staining was conducted with the anti-human FOXP3 (clone 206D) staining kit according to manufacturer recommendations (Biolegend).
Peanut Agglutinin (PNA) staining was conducted on cryo-sections with PNA-Alexa Fluor 488 conjugate (1∶250).
