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The slides were stained simultaneously to avoid staining variations, and the images were obtained in the same microscopic/camera configuration.
Different data sets may have varied properties which may be attributable to staining variations, inconsistent image acquisition settings, and image preparation.
By using tissue array, staining variations between samples were minimised and positive samples could be used as supplements for internal controls.
The variable 'goodness-of-fit' is a very robust texture analysis feature, since it is much more independent of staining variations than the FD [111].
These staining variations were confirmed in previous glioblastoma studies [ 58].
Automated image analysis of histology images is affected by the staining variations in histology slides.
Similar(52)
Like any experiments, there are some degrees of variations due to expression variation, staining variation or both.
The observed staining variation between DL-101 and B-A38 was observed on myeloma cell lines characterized for their bright or weak expression of CD138 molecules.
In order to reduce the noise level due to staining variation, median filter was used and, simultaneously, the image contrast was enhanced using histogram equalization technique and 2(b)).
To minimize staining-to-staining variations, staining experiments always included an equal amount of control and kindled samples.
However, the complex cellular structure, presence of imaging artifacts, uneven staining variation make the task challenging.
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