Sentence examples for staining transfer from inspiring English sources

Prior to staining, transfer sections to de-ionized water.

They were either visualised by Coomassie Brilliant Blue R250 staining, transferred to PVDF membranes for NH2-terminal microsequencing or transferred to nitrocellulose membranes for Western blot analysis as described elsewhere [ 25].

Equal loading of the gels and RNA integrity was determined by visualization of SYBR-gold(Invitrogen) stained gels prior to blot transfer.

We stained transferred membranes with Ponceau (Sigma) and scanned them with Canoscan LiDE70 (Canon, Lake Success, NY).

The PVDF membrane was dried and stained with SimplyBlue Safe Stain (Invitrogen) after western blot transferring.

These cells were stained for CD4, CD44 and CD62L and transferred cells in donor splenocytes were identified based on the dyes used for staining before transfer.

12 μl of the concentrated supernatants were applied and equal protein loading was confirmed by Ponceau staining after transfer onto nitrocellulose membranes.

Liver total lysates, high salt extracts or K8/K18 immunoprecipitates were resolved by 10% SDS-PAGE and then followed by either Coomassie Blue staining or transfer to PVDF membranes for immunoblotting.

Similarly, for rectal cancer a negative correlation has been observed between MVD (using CD31 staining) and transfer from the blood to EES (Ktrans) at DCE-MRI (Atkin et al, 2006), suggesting that similar physiological mechanisms related to dysfunctional tumour microvasculature may exist for other tumour types as well.

Samples were subjected to electrophoresis through 0.6% w/v agarose in 1× TBE overnight at 33 V, visualized after ethidium bromide staining and transferred to a GeneScreen™ hybridization transfer membrane (PerkinEilmer) in 10× SSC.

Oil from the stain will transfer to the paper bag or melt on to the wax paper.