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To verify whether the presynaptic markers were present in comparable amounts in healthy and dilute muscles, we undertook a statistical analysis of the BGT-stained areas covered by either synaptophysin or neurofilament M staining (Table 2).
Cutoff percentages for dichotomization of the data were determined for cyclin D1 and cortactin using the median percentage of stained cells; 23% for nuclear cyclin D1 and 13% for cytoplasmic cortactin staining (Table 1).
In contrast, 14/26 (54%) of ccRCCs had either 0 or 1+ mitochondrial staining (Table 1).
The chRCCs in this series demonstrated overall relatively high mitochondrial staining (Table 1) and relatively low MDR staining (Table 2), suggesting that these lesions should have high 99mTc-sestamibi uptake and should visually be indistinguishable from oncocytomas and HOCTs.
It is noteworthy that all of the localized cases of chRCCs had 3+ mitochondrial staining (Table 1).
For cryopreservation, the seeds stored in LN for one week showed 96% TTC staining, and seeds with no LN treatment showed 77% staining (Table 2).
This astrogliosis observed at the immunohistological level was, however, not reflected in the quantification of GFAP staining (Table 1).
Although MCM2 antibody recognized slightly more positive cells than Ki-67, we did not observe MCM2 positive cells in animals with no Ki-67 staining (Table 1).
All tumors examined by immunohistochemistry (n = 12) lacked nuclear staining for CDKN1C and showed negligible cytoplasmic staining (Table 1 and Supplementary Figure 5B).
These data, combined with the homogenous GFAP staining and the absence of NSE/synaptophysin staining (Table 2 and Figure 2), suggest that low-grade glioma cells are reminiscent of glial-progenitor cells.
were used for intracellular staining (Table 1).
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