Sentence examples for staining surface from inspiring English sources

Mean Fluorescence Intensity (MFI) values for HSPs and GR were measured after staining surface antigens CD33 and CD45 with CD33-PE/Cy5 and CD45-PE/Cy7 followed by intracellular staining with HSP72-FITC, HSP90a-PE or GR-FITC using 4-colour flow cytometry.

PrioV3 displayed binding specificity for both PrPC and PrPSc and was believed to bind PrPC in the cytosol of neurons (Tayebi et al, submitted); In marked contrast, conventional anti-prion antibodies produced in mouse against similar target antigen were unable to enter the neuronal plasma membrane and instead decorated the cell membrane by staining surface PrPC (Tayebi et al, submitted).

First, we are optimizing flow cytometry protocols and reagents to directly quantify antigen-specific B cells by staining surface IgG with labeled antigen.

At this stage, a maximal number of DSBs can be detected by staining surface spreads with an antibody against DMC1, which is a DNA-binding protein of DSBs.

To further investigate gene transcriptional activity specifically at pachytene we used gravity sedimentation to isolate stage enriched cell populations of at least 70%% pachytene purity assessed by SYCP1 staining surface spreads from each fraction (Fig.  6b and Additional file 2: Figure S2).

For intranuclear Foxp3 staining, surface stained cells were fixed and permeabilized using commercially available reagents (eBioscience) and stained with anti‐Foxp3 antibody.

To stain surface markers, cells were washed and stained in ice-cold staining buffer (2% FCS, 0.1% NaN3 in PBS).

Failure to stain surface CD24, or CD24-negativity (CD24neg), was defined by the gate set from unstained controls.

Mucous cells in all locations stained (surface and all levels of the foveolar pit) but with a tendency for a higher percentage of surface cells to be positive.

To stain surface antigens, cells were incubated with antibodies against CD10, CD15s, CD44, CD146 and CD282 for 30 min on ice.

Any paint or decorative element on the glass may run, smudge off or stain surfaces.