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Equal-sized areas placed in the same region were used to measure intensity, with background staining subsequently subtracted.
For comparison, the proteins were also detected by Sypro Ruby (a fluorescent dye for protein gel staining) subsequently on the same gel, as shown in Figure 2B.
Our immunohistochemical staining subsequently showed reduced expression of UGT1A in 145 urothelial neoplasms, compared with paired nonneoplastic urothelial tissues, as well as inverse correlations between UGT1A levels and tumor grade or pT stage [ 110].
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The section was stained subsequently either with hematoxylin/eosin (HE) or with DAPI and subjected to microscopy.
In dental practice, mechanical approaches, such as polishing and brushing with abrasive-based prophylactic pastes and toothpastes, are used to remove extrinsic tooth surface stains subsequently providing a whitening effect.
ThioS-staining subsequently confirmed that these deposits contained amyloid signatures.
More importantly pellicle coated surfaces exposed to protein denaturants or chlorhexidine did not stain when subsequently exposed to salts of iron and tin.
After one month, organ cell suspensions were prepared by mechanical disruption, stained, and subsequently analyzed by flow cytometry.
Subsequently, staining for CD8, CD69 and intracellular IFNγ was carried out according to the Fast Immune protocol (Pharmingen).
Semithin section preparations were made by cutting 0.5 μm sections through epon-embedded embryos and subsequently staining with toluidine blue.
AP staining was subsequently used to classify ESC-like colonies as undifferentiated or differentiated.
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