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loss of sample during entry into the IEF gel, efficiency of transfer from first to second dimension, protein loss during staining, staining efficiency, a protein's staining curve over time, staining curve over concentration, and dye bleaching.
However, the relation between original protein quantity in the sample and measured spot intensity is influenced by various intervening factors: loss of sample during entry into the IEF gel, efficiency of transfer from first to second dimension, protein loss during staining, staining efficiency, a protein's staining curve over time, staining curve over concentration, and dye bleaching.
Hematoxylin and eosin staining staining showed that a few epithelial cells in the fat pads were left after the treatment, surrounded by a large amount of fibrous tissue (Fig. 4b).
To determine the amount of Luxol fast blue staining that was due to myelin staining, staining intensity in the ROIs was subtracted from staining intensity values from the stratum radiatum of the hippocampus that contained the umyelinated Schaffer collateral.
For CD44s staining, staining intensities were scored as no staining (0), weak staining (1+), moderate staining (2+), or strong staining (3+).
The intensity of nuclear staining was scored for individual tumor cell nuclei as negative /no staining, staining weakly, staining intermediately, or staining strongly.
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Masson's staining stained the collagen fibers of the tissue green.
It has affected me greatly as it has stained stain my career.
Prepared sections were stained with HE staining, Masson staining, and toluidine blue staining.
Hematoxylin and eosin (HE) staining, Masson staining, and toluidine blue staining were performed.
(A) Safranin O staining; (B) toluidine blue staining; (C) alcian blue staining.
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