Exact(16)
Pollen grains were immersed in various staining solutions.
However, vital cells were negatively stained for both staining solutions.
Concentrated staining solutions and/or over-incubation would result in dark background filling the space between chromatids, whereas diluted staining solutions and/or a limited incubation period creates indistinguishable chromosomal spreads.
Filters were fixed in methanol and stained using Diff Quick staining solutions.
However, staining solutions may react with cellular components, such as RNA, potentially adversely affecting RNA integrity.
The plant tissues were incubated with GUS staining solutions at 37°C for 24 hrs.
Similar(44)
JC1 staining solution was prepared by diluting the stock in culture medium at 1 10 dilution ratio.
SYBRGold gel staining solution was purchased from Molecular Probes Inc. (Oregon, USA).
Following treatment, cells were stained with a crystal violet staining solution [46].
An additional 10 µl of staining solution was added and mounted with a coverslip.
The samples were incubated in GUS staining solution at 37°C overnight.
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