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Staining solution was discarded and the plate was washed by dipping three times in MilliQ water.
SYBRGold gel staining solution was purchased from Molecular Probes Inc. (Oregon, USA).
The seedlings were then immersed in GUS staining solution and treated as described above.
After an incubation time of 55 seconds, further 300 µl of staining solution were added.
Following treatment, cells were stained with a crystal violet staining solution [46].
An additional 10 µl of staining solution was added and mounted with a coverslip.
The samples were incubated in GUS staining solution at 37°C overnight.
Cell viability was tested using 7AAD viability staining solution (eBioscience).
Staining solution was added after discarding the 1% BSA.
DAPI staining solution: Dilute DAPI stock solution 1 1000 with 1 × PBS.
DAPI staining solution was purchased from Beyotime Institute of Biotechnology (China).
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