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Antibody specificity was confirmed by staining skin of AR-TFM mice, which lack functional ARs.
Oil red staining skin histology was performed to label lipids from liposomes in different skin layers.
Upon staining skin sections with H&E, we observed that ADF−/− CFL1+/+ or ADF+/+ CFL1−/− skin appeared normal and similar to wild-type (WT) ADF+/+ CFL1+/+ skin.
For all immunofluorescence staining, skin samples were directly embedded in O.C.T. compound, flash frozen, and stored at -80°C.
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Given an RGB image I l, the red channel R l is selected for the epidermis coarse segmentation, since the red channel of the H&E stained skin histopathological image provides good distinguishable information [22].
Be careful and wear gloves when applying as this does stain skin.
Shoe polish is usually flammable, can be toxic, and, if misused, can stain skin.
We stained skin further for other molecules involved in cell cycle progression such as PCNA and KI67 (see Fig. S2).
(A ) H&E stained skin sections of mice treated three times with acetone or TPA.
To further examine the expanded cell population after ADF/CFL1 loss, we stained skin sections with markers of differentiation.
To better understand the effects of Ets1 over-expression on the proliferative basal keratinocytes, we stained skin sections for a series of markers.
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