After 24 hours of serum starvation (0.2% FBS) BAMBI staining was reduced, whereas LC3 staining shifted towards an increase of a punctate pattern.
In the D36 mice (panels 2 and 5) and KO mice (panels 3 and 6), the RIIα staining is shifted in the dendritic fields and becomes concentrated in the cell body area of the pyramidal neurons (Fig. 2, red arrows in panels 1 9 indicate the pyramidal cell layer).
found that I2PP2A co-localized with PP2A and Rec8 in meiosis II cells, but I2PP2A staining was shifted away from PP2A and Rec8 signals in meiosis I cells [8].
Consistent with this notion, caveolin-1 staining appeared to shift from the leading process tip to a more even distribution throughout migratory cells after MDC treatment (not shown).
However, in the presence of differentiation medium, we neither observed the small fraction of cells that strongly expressed CD133 (0.06%+/−0.012; p>0.05 versus isotype staining) nor the shift in the average GMFI value of CD133.
If you have a really tough to shift stain on something white, the careful application of bleach can help.
Dot plot representative examples of these analyses are shown on Figure 1A, where each dot represents an individual cell, and an decrease in MHC staining results in a shift to the left, and an increase in MeCP2 in an upward shift.
Western blot analysis on total cell lysates containing β-ME confirmed ISGylation of UbcH10, H13 and H17 with AgmISG15 but not with HuISG15, as seen by a 15 kDa shift upon staining with anti-V5 Ab detecting the ectopic expressed UbcH proteins (Figure 3a,b and Figure S2a).
Examination of the amiR-cad1 stems revealed a reduction in red staining and a slight shift to yellow-orange color, notably in the epidermal cells and sclerenchyma fibers.
Incubation of IgM with wt Cc5 bacteria determined a dramatic reduction of SNA staining and a size shift of the IgM heavy chain indicating deglycosylation while incubation with PUL5 mutant bacteria did not affect the glycosylation state of IgM showing that IgM deglycosylation by Cc5 is only PUL5 dependent.
