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Intracellular stainings for Foxp3 were performed using the Foxp3 Staining Set (Natutec, eBiosciences) according to the manufacturer's instructions.
Slides were stained for differential leukocyte identification using a Hemacolor Staining Set (EMD Millipore, Gibbstown, NJ, USA).
FoxP3 staining set was purchased from ebiosciences (USA).
Foxp3 was analysed using the PE anti-mouse Foxp3 Staining Set (eBioscience, USA).
Foxp3 intracellular expression was detected using APC anti-human Foxp3 Staining Set from eBioscience, according to their standard protocol.
Intracellular staining of Foxp3 was performed using APC mouse/rat Foxp3 staining set (eBioscience, San Diego, USA).
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Four-color flow cytometry and the eBioscience PE and APC FOXP3 staining sets with modified eBioscience staining protocols were used.
Letting a stain set or washing and drying a stained article of clothing without pre-treating it first can result in a permanent stain.
Be as prompt as you can in taking clothes to the dry cleaner or laundry before stains set in permanently.
Parasitemia was monitored daily by thin blood smear stained with modified Giemsa stain (Protocol Hema3 Stain Set, Sigma, Oakville, ON).
Then, the methanol was removed followed by addition of 200 μL/well of 0.1% CV (Gram-staining set for microscopy; Merck) and the plates were incubated for 5 minutes.
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