Sentence examples for staining sequences from inspiring English sources

Rabbit or mouse IgG was used to ensure that there was no cross-reactivity between the two staining sequences.

Four to six common V. vinifera cultivars were used as an internal control and to ensure allele calls were consistent with samples run on silver stained sequencing gels.

A denaturing solution was used to denature immunoreagents of the first staining sequence.

Next, a second HIER step was applied to remove the antibodies from the first staining sequence, but leaving the deposits of blue reaction product unchanged [22].

Next, all immuno-reagents used in the first staining sequence were removed by a second heat-induced epitope retrieval step (10 min, 98°C), leaving the blue reaction product intact.

After the primary staining sequence, slides were rinsed in TBS and incubated in a double-stain blocking solution for 3 min (Dako cytomation) and then rinsed in TBS.

All sections were counterstained with Mayer's hematoxylin, and negative controls were included in each staining sequence and obtained by omitting the primary antibodies.

Each block was processed in paraffin, cut into 4 μm sections, and then, the proximal end of one slice per block was stained in sequence with hematoxylin and eosin 1%% (H&E).

In these controls we did not observe any interaction between the reagents of the first and the second sequences of staining.

They were then postfixed in 4% paraformaldehyde in PBS with 1% SMB for 20 minutes in order to deactivate the antibodies of the first sequence of staining.

All PCR products of positive reactions by agarose gel electrophoresis with ethidium bromide staining were sequenced completely in both directions for confirmation of sequence specificity.