Sentence examples for staining sequence from inspiring English sources

A denaturing solution was used to denature immunoreagents of the first staining sequence.

Next, a second HIER step was applied to remove the antibodies from the first staining sequence, but leaving the deposits of blue reaction product unchanged [22].

Next, all immuno-reagents used in the first staining sequence were removed by a second heat-induced epitope retrieval step (10 min, 98°C), leaving the blue reaction product intact.

All sections were counterstained with Mayer's hematoxylin, and negative controls were included in each staining sequence and obtained by omitting the primary antibodies.

After the primary staining sequence, slides were rinsed in TBS and incubated in a double-stain blocking solution for 3 min (Dako cytomation) and then rinsed in TBS.

Four to six common V. vinifera cultivars were used as an internal control and to ensure allele calls were consistent with samples run on silver stained sequencing gels.

Rabbit or mouse IgG was used to ensure that there was no cross-reactivity between the two staining sequences.

Each block was processed in paraffin, cut into 4 μm sections, and then, the proximal end of one slice per block was stained in sequence with hematoxylin and eosin 1%% (H&E).

Mycobacterial isolates were identified by their phenotypic properties (temperature preference, growth rate, and pigment production in the dark and upon exposure to light), microscopic examination after ZN staining, and sequence analysis of the 16S rRNA gene [ 9].

They were then postfixed in 4% paraformaldehyde in PBS with 1% SMB for 20 minutes in order to deactivate the antibodies of the first sequence of staining.

In these controls we did not observe any interaction between the reagents of the first and the second sequences of staining.