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The use of Ru stained poly styrene) film as masks for cryogenic plasma etching can be beneficial since at the staining saturation, the overall etch rate is divided by 7 compared to unstained film and the selectivity Si PS increases from 10 1 to 70 1.
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As staining intensity demonstrates a saturation effect, the concentration developing a moderate staining intensity (about half of the maximum intensity of a blank sample) was further used.
In addition to the proportion of cells stained, the saturation of the stain is also measured, where a deeper stain indicates a greater intensity of the receptor.
For instance, it is possible that some glycolytic enzymes were not considered in this analysis because of the pI or the molecular weight ranges employed, comigration phenomena and problems of saturation staining.
A fluorescence decrease related to microalgae cell concentration was shown below a minimum threshold and above a maximum threshold of cell concentration for lipid staining, where saturation occurs.
Camera acquisition parameters were determined such that no image contained pixels at saturation and that cells in cultures stained with only secondary antibody only minimally registered, thereby establishing the dynamic range for measurement from background fluorescence to maximal staining intensity.
Saturation was confirmed by staining with dilutions of antibodies on each set of cells, except for human ex vivo cells, for which saturation was confirmed by staining WIN cells.
Staining variability was corrected by digitally adjusting color saturation.
Hue Saturation and Brightness (HSB) segmentation ranges for DAB brown staining and hematoxylin alone (cells not staining brown) were defined by creating different color masks.
After the background staining correction, the optical density was calibrated and the area of interest was set through: hue, 0 30; saturation, 0 255; intensity, 0 230.
Staining for BCL-X and BAX was allowed to continue until one of the comparative strips had reached saturation.
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