Sentence examples for staining quantity from inspiring English sources
For immunohistochemical assessment of EZH2 expression, nuclear staining quantity was evaluated using a semiquantitative score.
Following amplicon separation on 2% agarose gel with ethidium bromide staining, Quantity 1 1-D analysoftwareware (BioRad, Hercules, California) determined band density.
The nuclear staining quantity was evaluated with percentages of cells stained in analogy to routine Ki-67 immunostainings in categories of 10% intervals.
RNA integrity was confirmed by ethidium bromide gel staining and quantities were determined spectrophotometrically [ 55].
We agree with the reviewer that additionally there are two alternative methods to address the internalization question as discussed above: (1) to use a fluorophore-conjugated version of NP-TGT, for example, Cy3-NP-TGT; (2) to use NP-specific antibody to stain the quantity of the NP-TGTs on the surface of the coverslip.
Staining techniques that measure the accumulated quantity of proteins can overlook minor changes in protein quantities because they disappear within noise or systematic errors.
After permeabilisation and staining, the quantity of cellular DNA is analysed by flow cytometric analysis, using the intensity of fluorescence of the stained DNA.
In benign nodules, positive staining of CD34 manifested that a smaller quantity of staining tumor capillaries were observed.
Several studies provided an immunoreactive score (IRS), which was calculated by multiplying a staining intensity score with a score for quantity of staining.
The evaluation of COX-2 expression by quantity of staining alone may allow more tissue to be regarded as positively stained, as this does not incorporate the intensity of staining like in other evaluation methods.
As shown in Fig. 3, panel F, MФs that were not pretreated with cytoD and were infected with CO92 pPst- pgm-, revealed bands corresponding to anti-YopM Ab staining (panel F-a), the quantity of which was reduced by the addition of cytoD (panel F-b), and eliminated when CO92 pPst- pgm- was pretreated with SMI before infection (panel F-c).
