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Evidence of positive staining was recorded as presence of staining (yes/no) or percent of epithelial or basal cells staining (number of cells staining over total number of cells).
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Staining indices (number of stained tumor cells per total number of tumor cells) were assigned semi-quantitatively to five categories: <20%, 20 39%, 40 59%, 60 79%, >80%.
We counted the total number of cells (DAPI staining), the number of neurons (beta-tubulin staining, also labeled by DAPI), and the number of Purkinje cells (calbindin staining, also labeled by beta-tubulin and DAPI).
After fixing and staining, the number of migrated cells was quantitated.
By HE staining the number of the cells was counted microscopically.
For all nuclear staining the number of positive cells were counted in 1000 cells in five high-power fields (hpf) and percentage derived.
21 The expression of CLCA1 was scored as the ratio of the number of positive cells to the total number of cells (stained cells/total number evaluated).
Stain number (r: 0.13, p=0.39), mean size (r: 0.24, p=0.25) and median colour intensity (r: 0.22, p=0.13) had no influence on measured FEV1 among cases.
After staining, the numbers of colony were counted.
The polyclonal anti-lysozyme antibody[ 34] stained a number of cells in the intestinal epithelium, for example, but we failed to confirm the specificity of the staining.
The total number of nuclei and total number staining at each intensity were counted.
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