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The HLA complex (peak 2) was analyzed by SDS-PAGE electrophoresis and coomassie blue staining (line 2).
In the 48 invasive bladder carcinomas, we observed two patterns of staining for collagen IV: in the first one, the staining line was conserved or partially fragmented (28 tumours), while in the second one the staining line was widely fragmented or absent in more than 5% of the tumour area (20 tumours).
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Cells located in the subgranular zone between dentate gyrus and hilus show clearly reduced staining in line with reduced hmC levels (Figure 6).
To study the influence of dye molecules, we compared the ablation probabilities both with and without Hoechst staining before line cutting.
Second, puzzles differ in the number and richness of extraneous features (e.g. different colors, stains, line markers) that add significantly to the difficulty.
Thus, we have demonstrated the utility of COIN for staining cell lines as well as primary human samples.
Samples were scored positive when both the test and control lines stained and negative when staining at control line was observed and the test line remained negative.
Antibodies were used at the same dilution as for the germ line staining.
What if I scratched it, or my lipstick stained the lining?
A comparison of staining for the lines G-28, U87 and G-112 using the two procedures is shown in Figure 1B,C.
In most lines, staining intensity was at least slightly increased after O- or N-deglycosylation.
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