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In contrast, tsBN67 cells already displayed a low level of H3S10P staining defects at permissive temperature and this level rose to nearly 20% at non-permissive temperature.
Under the light microscope, at 250× magnification, 30 non-overlapping fields were randomly selected for each section, avoiding areas with holes, folds, staining defects or large vessels.
Given the evident changes in C. elegans H3S10P detection upon loss of the Ce HCF-1 protein, we asked whether mammalian cells lacking HCF-1 function also display H3S10P staining defects.
As noted above, the percentage of embryos with H3S10P staining defects increased with worms grown at 12°C than at 20°C (about 70%, vs. 55%, see Fig. 6).
Interestingly, E17.5 Snrk KO hearts do not show lipid staining defects but P0 hearts do.
The stress sensitivity and iodine staining defects in these cells may reflect an influence of lcf2+ on the activity or delivery of the integral membrane Mok14 alpha-glucan synthase responsible for synthesis of the iodine-reactive polymer (Garcia et al. 2006).
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The CPY*-D1 and CPY*-D2 variants exhibited no detectable extra-endoplasmic reticulum staining indicating defects in ER export.
(20) Mucin-depleted foci (MDF) are the stain-defect lesions by periodic acid Schiff (PAS), which readily detects mucin.
At permissive temperature (33.5°C), BHK21 cells did not display any mitotic H3S10P-staining defects and only a low level (about 2%) of defective H3S10P staining at non-permissive temperature (40°C).
After the stimulation of mucin secretion by exposure to an ATP aerosol, Het mice appeared to release only half as much intracellular mucin as WT mice by PAFS staining, indicating a defect in stimulated secretion, though image saturation of the unstimulated airways precludes the calculation of fractional mucin release.
Specialized ophthalmologists collected clinical data by slit-lamp examination (using corneal fluorescein staining to study epithelial defects) and evaluated epithelial defects, inflammation, corneal opacity, and corneal neovascularization.
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