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To ensure staining consistency and reproducibility, a number of precautions were taken: (i) All sections from different blocks were stained as a batch on the same day and the process was repeated four times for each protein; and (ii) results were analysed independently by the three observers (EB, JCC and KJR).
To maintain staining consistency, paraffin-embedded sections (5 µm thick) were immunostained with a DAKO-Autostainer using a primary antibody to human GRN (anti-human progranulin, 1 600, R&D Systems, Inc., Minneapolis, MN) and 3, 3′-diaminobenzidine as the chromogen.
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Watercolor paints may also be either non-staining or staining.
And consistency.
Good consistency.
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