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After Ziehl-Neelsen staining confirmation of positive cultures, the isolates were identified using partial rpoB sequencing [ 10].
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Positive cultures were subjected to Ziehl-Neelsen (ZN) staining for confirmation of mycobacterial growth, and isolates were later confirmed as MTC at the molecular level by a previously described PCR typing panel [ 4].
Positive samples were removed from the machine and subjected to ZN staining for confirmation of the presence of mycobacteria, but no attempt was made to confirm the species.
(B ) Example coronal brain slice magnification using cresyl violet staining for confirmation of electrodes position.
Positive cultures were subjected to AFB staining for confirmation of mycobacterial growth.
Negative (omission of antibody) and positive controls (oral cancer) cases were run to verify the quality of staining and confirmation of the procedures.
Sputum smear examination was performed at the research project laboratory using fluorescence microscopy with auramine staining, with confirmation of positive smears by light microscopy using Ziehl-Neelsen (ZN) stain.
The hemadsorption test result was positive for all isolates with subsequent confirmation by immunofluorescence staining.
1 The differential diagnosis of SDH-deficient renal carcinoma, which includes oncocytoma and chromophobe renal carcinoma is limited, and we consider loss of staining for SDHB as definitive confirmation of the diagnosis.
Acid-fast staining was performed with an auramine-rhodamine fluorescent stain, followed by confirmation with Ziehl-Neelsen staining.
In case of positive p16 staining, a linear array analysis for confirmation was followed.
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