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Compared with the conventional protocols for in-gel protein staining, such as SYPRO Ruby and silver staining, compound 1 achieves higher sensitivity, even though it offers a simplified, higher-throughput protocol.
The nuclear staining compound ToPro3 was visualized by excitation at 642 nm and emission at 661 nm.
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These accessions were selected primarily because of the high intensity of darkly staining compounds present in preserved haustoria (compared to other accessions with less intensely darkly stained haustoria), which would provide a robust test of the protocol's efficacy.
Stained compounds were assayed photometrically at λ= 370 nm, against 2 N HCl (UV vis spectrophotometer Jenway 6315).
Damage can occur because of mechanical stress, production of staining compounds or enzymatic action (Blyskal 2009; López-Miras et al. 2013; Pinzari et al. 2010; Santos et al. 2009; Sterflinger 2010).
Ceric-sulphate in 10%H2SO4O4 spraying reagent was used for staining the compounds on TLC.
T5 also proved to be the most consistent treatment of the experiment, staining secondary compounds with similar colors and contrast uniformly in all the species.
Figure 2 The Hoechst 33258 staining of compound 3c in tumor cells.
Figure 1 The AO/EB staining of compound 3c in tumor cells.
Accordingly, phospho-S6 staining in compound Pml−/− Tsc2+/− mice colocalized with the same markers (Supporting Information Fig 1D, lower panels), demonstrating that mTORC1 upregulation in mutant mice occurs in the distal tubules and/or collecting ducts.
The hoechst staining assay showed that compound T10 caused morphological changes.
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