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Purified spinae were examined with SDS-PAGE on 12.5% gels (A, sizes of protein markers on left) or by TEM (negatively stained) without treatment (A, lane 1 and B), or digested with proteinase K at a concentration of 2 mg/ml (A, lane 2; C10, 10 mg/ml (A, lane 3; C2) and 20 mg/ml (A, lane 4; C3), or heated at 100°C for 1 min (D1), 5 min (D2), 10 min (D3 and D4).
The negative controls were sections stained without primary antibodies.
In addition, 9 cases of benign prostatic hyperplasia were stained without evidence of c-erbB-2 expression detected.
In the last few years, this imaging technique has progressively evolved from the simple staining of the common samples used in pathology for HC/IHC to the direct application on tissues that can be stained without additional procedures.
Control sections for each antibody were stained without primary antibody.
For each experiment, a section was stained without primary antibody to serve as a negative control.
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Staining without applying a first antibody served as negative control.
Isotype controls and staining without primary antibody were used as controls.
Controls included staining without primary antibodies.
Staining without the peptide served as a negative control.
Staining specificity was confirmed by a series of negative control staining without primary antibodies.
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